Part:BBa_K1582017
LC+sJanus-m Fusion Protein
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1141
Illegal AgeI site found at 600 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1150
Usage
This fusion protein is designed to degrade PET into terephthalic acid and ethylene glycol more powerfully. Compared to single LC, the rate of PET emzymolysis is supposed to be improved by LC-Janus fusion.
Biology
LC is a kind of cutinase, the details of which can be seen at BBa_K936000. Class II hydrophobins sJanus are small secreted fungal proteins which can be found in filamentous fungi, which play a role in a broad range of processes in the growth and development of filamentous fungi. Their assembly shows thread-like structure. They could be expressed in prokaryotic cells like E.coli. We did some mutations to it, and we call it sJanus-m.
According to some research, when Janus is added to the reaction of cutinase and plastics, the reaction rate is supposed to have an obvious improvement.
Based on the above background, we design to use the method of fusion protein to combine cutinase and Janus compactly, through the establishment of fusion protein, we can found if we can further promote the stimulation rate of the plastics degradation. Meanwhile, from the data about fusion protein, we can try to uncover the functional mechanism of the stimulation to the plastics degradation which is unknown until now.
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